Cloning expression of a novel human protein-disulfide isomerase like cDNA.
- Author:
Jin HUANG
1
;
Lei YANG
;
Yulan ZHANG
;
Xuanmao CHEN
;
Kang YING
;
Yi XIE
Author Information
1. College of Medicine, Shihezi University, Xinjiang
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Base Sequence;
Cloning, Molecular;
DNA, Complementary;
genetics;
Gene Library;
Humans;
Protein Disulfide-Isomerases;
biosynthesis;
genetics
- From:
Journal of Biomedical Engineering
2002;19(3):459-462
- CountryChina
- Language:Chinese
-
Abstract:
Using the large scale sequencing, a novel human cDNA of 1,645 bp was screened from the cDNA library of human fetal brain. The cDNA contains an ORF encoding a 296-aa protein with a calculated molecular weight of 34.0 KD. Compared with that of current sequence databases, the putative protein was found to have 36% homology with protein-disulfide isomerase (PDI). So this cDNA was named PDI like (PDI-L) gene. Multiple tissue Northern blot analysis shows that PDI-L cDNA is expressed in heart, brain, liver, kidneys and so on. The ORF fragment of PDI-L cDNA was inserted into reconstructed pBV220 expression vehicle and its predicted expressive protein was obtained.
