Effect of microsomal triglyceride transfer protein gene polymorphism in the promoter region on dyslipidemia in type 2 diabetic subjects.
- Author:
Liming CHEN
1
;
Gen YOSHINO
;
Eiichi MAEDA
;
Shufan ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Carrier Proteins; genetics; Diabetes Mellitus, Type 2; genetics; Genotype; Humans; Hyperlipidemias; etiology; genetics; Polymorphism, Genetic; Promoter Regions, Genetic
- From: Chinese Medical Journal 2003;116(2):215-217
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the relationship between microsomal triglyceride transfer protein (MTP) gene variation and diabetic dyslipidemia among Chinese.
METHODSUsing PCR restriction fragment length polymorphism (PCR-RFLP) analysis and gene sequencing, we studied the influence of a common MTP gene polymorphism in the promoter region on the apoB-containing lipoproteins in 44 Chinese type 2 diabetic subjects and 32 non-diabetic volunteers.
RESULTSA common functional G/T polymorphism in 493 bp upstream from the transcriptional start point was detected among native Chinese. There were 41 carriers (53.9%) of the MTP-493 G/G genotype, 28 (36.8%) of the MTP-493 G/T genotype and 7 (9.3%) of the MTP-493 T/T genotype. The allele frequency of MTP-493 T in the diabetic group was 0.30. The MTP-493 T/T diabetic group had significantly higher TG (P < 0.05), VLDL-CH (P < 0.05) and smaller LDL particle size (P < 0.001) than the MTP-493 common genotype group.
CONCLUSIONGenetic variation in the MTP promoter is likely to be highly involved in the production of dyslipidemia in type 2 diabetic subjects.
