Experimental study on apoptosis in leukemia cells induced by econazole.
- Author:
Fang LIU
1
;
Ping ZOU
;
Ming ZHANG
;
Yao-Hui WU
;
Juan XIAO
Author Information
1. Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
drug effects;
Blotting, Western;
Calcium;
metabolism;
Caspase 12;
metabolism;
Caspase 7;
metabolism;
Cell Line, Tumor;
Dose-Response Relationship, Drug;
Econazole;
pharmacology;
Flow Cytometry;
Leukemia;
metabolism;
pathology
- From:
Journal of Experimental Hematology
2005;13(3):391-393
- CountryChina
- Language:Chinese
-
Abstract:
To investigate apoptosis of mouse leukemia cell (WEHI-3) induced by econazole and its mechanism, apoptosis induced by econazole was examined by flow cytometry, while free calcium ([Ca(2+)]i) was determined by Fura-2 fluorescein load technique. The protein was isolated from endoplasmic reticulum of WEHI-3 cells, and then the expression of caspase-12 and caspase-7 was evaluated by Western blot. The results showed that WEHI-3 exhibited typical change of apoptosis when it was treated by econazole, [Ca(2+)]i was significantly higher in comparison with the control. The expression of caspase-12 and caspase-7 enhanced as the econazole concentration increased. In conclusion, econazole can induce WEHI-3 cell apoptosis and the caspase-12 plays a key role in this process.
