Treatment of premenstrual syndrome with Chinese medicine formula, baixiangdan capsule: implications for neuron viability and GABA(A) receptor modulation in rat cortex.
- Author:
Shiguang SUN
1
;
Mingqi QIAO
;
Huiyun ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Acetophenones; administration & dosage; Administration, Oral; Animals; Benzoates; administration & dosage; Bridged-Ring Compounds; administration & dosage; Cell Culture Techniques; Cell Proliferation; Cell Survival; drug effects; Cerebral Cortex; metabolism; pathology; Disease Models, Animal; Drugs, Chinese Herbal; administration & dosage; Female; Glucosides; administration & dosage; Liver Diseases; drug therapy; Monoterpenes; Neurons; drug effects; metabolism; pathology; Patch-Clamp Techniques; Premenstrual Syndrome; drug therapy; Qi; Rats; Rats, Wistar; Receptors, GABA-A; drug effects; metabolism
- From: China Journal of Chinese Materia Medica 2011;36(11):1508-1511
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of Baixiangdan capsule (BXD), a Chinese medicinal formula, on the premenstrual syndrome (PMS) rats with Liver-qi invasion and the possible underlying micro-mechanisms.
METHODWistar rats were randomly assigned to two groups, nomal group and BXD group After evaluated by macro-behavior observation and open-field test, MTT assay and the whole-cell patch clamp recording were performed respectively to evaluate the effects of serum from BXD capsule-treated rats on the viability and GABA(A) R-induced currents of cortical neurons in vitro.
RESULTIn the open-field test, the crossing score, rearing score and total score of BXD rats decreased significantly (P < 0.05), compared by the normal rats. Compared with cells exposed to serum of normal rats, the viability values of those incubated with serum of BXD group for 24 h and 48 h significantly increased (P < 0.05), measured by MTT assay. The results of whole-cell patch clamp recording showed that concentration-response relationship curves revealed an EC50 value of (29.0 +/- 4.4) micromol x L(-1) and a Hill coefficient of 1.07 for normal-exposed cultures, (63.5 +/- 8.2) micromol x L(-1), 1.04 for BXD-exposed cultures after incubation for 24 h. Furthermore, the difference in EC50 values was statistically significant (P < 0.01), that in the Hill coefficient was not obvious.
CONCLUSIONBXD capsule could significantly decrease crossing score and total score of open-field test and effectively enhance the neuron viability and GABA(A) receptor activity in rat cortex. Paeonimetabolins I and paeonol may play a significant role in treating PMS model rats with Liver-qi invasion by BXD capsule, and paeonol may target at GABA(A) receptor, especially.
