Tetrahydropalmatine's permeative properties of acupoint and non-acupoint transdermal administration of Baijiezi Tufang in vitro and in vivo.
- Author:
Xiucai GUO
1
;
Xia LIU
;
Yuehong XU
Author Information
- Publication Type:Journal Article
- MeSH: Acupuncture Points; Administration, Cutaneous; Animals; Berberine Alkaloids; administration & dosage; pharmacokinetics; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; administration & dosage; metabolism; Guinea Pigs; Skin; metabolism
- From: China Journal of Chinese Materia Medica 2012;37(7):1034-1038
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study tetrahydropalmatine's permeative properties of acupoint and non-adupoint transdermal administration of baijiezi tufang in vitro and in vivo.
METHODTaking tetrahydropalmatine as an evaluative component to assess the permeative of baijiezi tufang in acupoint skin and non-acupoint skin with the modified Franz diffusion cell method and in vivo penetration studies. The content of tetrahydropalmatine was determined by a HPLC method.
RESULTThe 24 hours cumulative permeation amount through acupoint skin was (13.53 +/- 3.92) microg x cm(-2), about 4 times higher than non-acupoint skin. The steady-state infiltration rates of tetrahydropalmatine in acupoint skin was (0.659 1 microg x cm(-2) x h(-1)), 4.5 times higher than non-acupoint skin. The content in acupoint skin was signally higher than that in non-acupoint skin (P < 0.05). An accumulation of fluorescence can be clearly seen in the four layers: stratum corneum > viable epidermis > dermis > subcutaneous.
CONCLUSIONIn vitro and in vivo studies show that the permeation of baijiezi tufang in acupoint skin was better than in non-acupoint skin, following a higher cumulative amount and skin content.
