Human soluble dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin inhibits phagocytosis of Staphylococcus aureus by immature dendritic cells.
- Author:
Hui-Jie LI
1
;
Tian-Yu XU
;
Jia ZHOU
;
Ling-Yan ZHU
;
Li-Yun ZHANG
;
Xiao LU
;
Zheng-Liang CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Cell Adhesion Molecules; metabolism; Dendritic Cells; cytology; metabolism; Humans; Lectins, C-Type; metabolism; Lipopolysaccharides; Phagocytosis; Receptors, Cell Surface; metabolism; Staphylococcus aureus; Teichoic Acids
- From: Journal of Southern Medical University 2015;35(4):544-548
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect and mechanism of soluble dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (sDC-SIGN) on the phagocytosis of Staphylococcus aureus (S. aureus) by immature dendritic cells (imDCs).
METHODSFlow cytometry was employed to examine the effect of sDC-SIGN on the phagocytosis of S. aureus by imDCs. Enzyme-linked immunosorbent assay (ELISA) was used to analyze the binging of sDC-SIGN to S. aureus, lipoteichoic acid (LTA) and lipopolysaccharides (LPS) and investigate the effect of the ligands mannan and LTA and anti-DC-SIGN antibodies 1C6 and 4H3 on the binging of sDC-SIGN to S. aureus.
RESULTSsDC-SIGN inhibited the phagocytosis of S. aureus by imDCs. sDC-SIGN bound to S. aureus in a Ca(2+)-dependent manner. sDC-SIGN concentration-dependently bound to LTA, but not to LTA, and the binging of sDC-SIGN to S. aureus was blocked by mannan, LTA, 1C6 and 4H3.
CONCLUSIONsDC-SIGN preferentially binds to the carbohydrate constituents on S. aureus to affect the binding between membrane-bound DC-SIGN and S. aureus, thus suppressing the phagocytosis of S. aureus by imDCs.
