Effect of indomethacin on BCR/ABL-Wnt/β-catenin pathway in K562 cells.
- Author:
Zhangling LIU
1
,
2
;
Jing HU
;
Zhenglan HUANG
;
Hui LI
;
Xin LIU
;
Wenli FENG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Cycle; Cell Proliferation; Fusion Proteins, bcr-abl; metabolism; Humans; Indomethacin; pharmacology; K562 Cells; drug effects; RNA, Messenger; Wnt Signaling Pathway; drug effects; beta Catenin; metabolism
- From: Journal of Southern Medical University 2015;35(7):998-1002
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of indomethacin on the proliferation and Wnt/β-catenin pathway in K562 cells.
METHODSThe cell growth of K562 cells treated with different concentrations of indomethacin was assessed with MTT assay, and the colony-forming ability of the cells was evaluated by colony-forming assay. The mRNA expressions of BCR/ABL and β-catenin were detected by RT-PCR, and the protein expressions of pBCR/ABL, total BCR/ABL, β-catenin, pGSK-3β and c-myc were analyzed by Western blotting.
RESULTSIndomethacin significantly suppressed the growth and colony-forming ability of K562 cells in a dose-dependent manner. Indomethacin treatment dose-dependently decreased the protein level of pBCR/ABL and total BCR/ABL without affecting bcr-abl mRNA expressions. Compared with the control groups, indomethacin-treated cells showed obviously decreased mRNA and protein expressions of β-catenin and decreased protein expressions of pGSK-3β and c-myc.
CONCLUSIONIndomethacin inhibits the proliferation of K562 cells by suppressing the activity of bcr-abl-Wnt/β-catenin pathway.
