Inhibitory effect of ZD6474 combined with adriamycin on MCF-7 human breast cancer cells in vitro.
- Author:
Jie SHEN
1
;
Ben-fu HE
;
Jian RUAN
;
Peng ZHAO
;
Jing-xia MIAO
;
Hang ZHENG
;
Rong-cheng LUO
Author Information
- Publication Type:Journal Article
- MeSH: Antibiotics, Antineoplastic; pharmacology; Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Breast Neoplasms; pathology; Cell Cycle; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Doxorubicin; pharmacology; Drug Synergism; Female; Humans; Piperidines; pharmacology; Quinazolines; pharmacology
- From: Journal of Southern Medical University 2010;30(3):523-525
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the killing effect of ZD6474 combined with adriamycin (ADM) on MCF-7 human breast cancer cells.
METHODSThe inhibitory effects of ZD6474 and ADM alone and in combination on the proliferation of MCF-7 cells were assessed by MTT assay. The cell cycle and cell apoptosis were detected by flow cytometry.
RESULTSZD6474 and ADM both significantly inhibited the proliferation of MCF-7 cells, showing a synergistic effect of their reactions in combined use (P<0.05). ZD6474 or ADM alone caused cell cycle arrest at G0/G1 and S phases, respectively. Combined use of the two drugs resulted in significant reduction of the M-phase cell percentage and cell cycle arrest at G0/G1 and S phases. The coadministration of the drugs significantly increased the apoptosis rate of the cells as compared with ZD6474 or ADM treatment alone (P<0.05).
CONCLUSIONSZD6474 and ADM show a synergistic effect in inhibiting the proliferation and inducing apoptosis of MCF-7 cells.
