Effect of hTERT antisense oligodeoxynucleotide on telomerase activity in bladder cancer cells in vitro.
- Author:
Xiao-dong GAO
1
;
Yi-rong CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Gene Expression Regulation, Neoplastic; Humans; Oligodeoxyribonucleotides, Antisense; pharmacology; RNA, Messenger; genetics; metabolism; Telomerase; genetics; metabolism; Urinary Bladder Neoplasms; enzymology; pathology
- From: Journal of Southern Medical University 2010;30(4):779-781
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of hTERT antisense oligodeoxynucleotide on telomerase activity in bladder cancer cells.
METHODSAntisense phosphorothioate oligodeoxynucleotide (AS PS-ODN) was synthesized and purified. Telomerase activity was measured by telomerase PCR ELISA kit. hTERT mRNA expression was detected by reverse transcription-polymerase chain reaction (RT-PCR), and hTERT protein by immunohistochemistry and flow cytometry.
RESULTSTelomerase activity was decreased in T24 cells 48 h after treatment with AS PS-ODN, and was significantly inhibited at 72 h.
CONCLUSIONAS PS-ODN can significantly inhibit telomerase activity by down-regulating hTERT mRNA and protein expression in bladder cancer cells.
