Comparative proteomics research of apoptosis initiation induced by homoharringtonine in HL-60 cells.
- Author:
Chun-yan CHEN
1
;
Ji-hui JIA
;
Xiang-lin PAN
;
Yue-sheng MENG
;
Zhen-hua TU
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; pharmacology; Apoptosis; drug effects; Calbindins; Chloride Channels; analysis; DNA-Binding Proteins; analysis; Electrophoresis, Gel, Two-Dimensional; methods; HL-60 Cells; Harringtonines; pharmacology; Histocompatibility Antigens Class I; analysis; Humans; Ikaros Transcription Factor; Inhibitor of Apoptosis Proteins; Microtubule Proteins; Phosphoproteins; analysis; Proteins; analysis; Proteome; analysis; S100 Calcium Binding Protein G; analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Stathmin; Transcription Factors; analysis
- From: Chinese Journal of Hematology 2003;24(12):624-628
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the related proteins of apoptosis initiation induced by homoharringtonine (HHT) in HL-60 cells.
METHODSAfter establishment of an apoptosis initiation model induced by HHT in HL-60 cells, proteins of untreated and HHT treated HL-60 cells were extracted, and the two-dimensional polyacrylamide gel electrophoresis (2-DE) maps of the extracted proteins were established by using the immobilized pH gradient (IPG) two-dimensional electrophoresis respectively. The alteration protein spots were identified with assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database searching.
RESULTSProteomics analysis showed that proteins including MHC class I antigen, calbindin D-28K, chloride channel protein 6, oncoprotein 18, zinc finger protein Helios and apoptosis inhibitor like protein 2 were involved in apoptosis initiation induced by HHT.
CONCLUSIONThe present study might conduce to the researches of HL-60 cells carcinogenesis and pave the way to exploit drug precursor related to HHT and initiation of apoptosis in HL-60 cells.
