Determination of three chemical constituents in zhuang medicine Blumea megacephala (Randeria) by HPLC.
- Author:
Xiaoqing NING
1
;
Yaohua LI
;
Yuanfeng TAN
;
Yong CHEN
;
Yan HUANG
;
Fuqi PAN
Author Information
- Publication Type:Journal Article
- MeSH: Asteraceae; chemistry; Caffeic Acids; chemistry; Chlorogenic Acid; chemistry; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; Hydroxybenzoates; chemistry; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2011;36(12):1623-1625
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for content determination of protocatechuic acid, chlorogenic acid and caffeic acid in Zhuang medicine Blumea megacephala (Randeria), and explore the content variation of the 3 components of the herbs harvesting in different months, and provide the scientific basis of reasonable application.
METHODThe determination was carried out on a Shimadzu VP-ODS column (4.6 mm x 250 mm, 5 microm) eluted with acetonitrile and water containing 0.1% phosphoric acid in a linear gradient elution. The flow rate was 1 mL min(-1), and the detected wavelength was set at 258, 327nm.
RESULTThe peak areas and the concentrations of the three components had good linear relationship in the range of 1.7-17 mg x L(-1) for protocatechuic acid, 15.6-156 mg x L(-1) for chlorogenic acid, 3.96-39.6 mg x L(-1) for caffeic acid. The average recoveries were 103.4%, 102.2%, 98.5%, respectively.
CONCLUSIONThe method was proved to be simple, accurate and used for the quality evaluation of Blumea megacephala (Randeria).
