Effect of polypeptide extract from scorpion venom (PESV) on expression of HIF-1alpha and SDF-1/CXCR4 in repopulating H22 tumour tissue during chemotherapy treatment.
- Author:
Zhaopeng WANG
1
,
2
;
Weidong ZHANG
;
Licun WU
;
Qing JIA
;
Zhaoxia WANG
;
Yueying ZHANG
;
Yunna NING
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Chemokine CXCL12; drug effects; metabolism; Down-Regulation; drug effects; Hypoxia-Inducible Factor 1, alpha Subunit; drug effects; metabolism; Mice; Peptides; pharmacology; Receptors, CXCR4; drug effects; metabolism; Scorpion Venoms; chemistry; pharmacology; Scorpions; chemistry; Time Factors
- From: China Journal of Chinese Materia Medica 2011;36(13):1803-1807
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of HIF-1alpha and SDF-1/CXCR4 in repopulating H22 tumor tissue and the mechanism of angiogenesis of polypeptide extract from scorpion venom (PESV) during chemotherapy treatment.
METHODThe expression of HIF-1alpha and SDF-1/CXCR4 in H22 tumor tissue was monitored by immunohistochemistry, and the expression level was determined by Qwin V3 image analyzing software. The correlation between HIF-1alpha and SDF-1 was analyzed. SDF-1 content was detected by ELISA.
RESULTHIF-1alpha expression was found no difference in model group between 14 d and 21 d, and up-regulated in 28 d. There was no change of HIF-1alpha expression was observed in low-dose PESV group. In high-dose PESV group, the level of HIF-1alpha expression was high in 14 d and low in 21 d. ELISA detecting showed SDF-1 content increased slowly from 14 d to 21 d, highly from 21 d to 28 d. But in high-dose PESV groups, the content increased slowly all the time. The immunohitochemistry method got the same result with ELISA. Correlation analysis showed r = 0.805. CXCR4 expression down-regulated in two PESV treated groups, and no difference was found between these two groups.
CONCLUSIONHIF-1alpha and SDF-1 participated in VEGF expression and angiogenesis in tumor tissue during chemotherapy, while PESV could inhibit the expression of HIF-1alpha and SDF-I.
