PI3-kinase mediates thrombin-induced platelet aggregation through mDia1 pathway..
- Author:
Guang-Xun GAO
1
;
Hong-Juan DONG
;
Hong-Tao GU
;
Ying GAO
;
Yao-Zhu PAN
;
Yi-Wei WANG
;
Yang YANG
;
Xie-Qun CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Actins; Animals; Blood Platelets; metabolism; Humans; Phosphatidylinositol 3-Kinases; Platelet Aggregation; Platelet Aggregation Inhibitors; pharmacology; Thrombin; pharmacology
- From: Chinese Journal of Hematology 2010;31(3):176-180
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of mDia1 (mammalian diaphanous 1)in platelet and the role of mDia1 or phosphatidylinositol 3-kinase (PI3K) in the process of thrombin-induced platelet aggregation.
METHODSThe extent of platelet aggregation was measured by a platelet aggregation system and the expression of mDia1 and its relation with F-actin in quiescent, spreading or aggregated platelets by Western blot.
RESULTSThere was no significant difference in mDia1 expression level between quiescent and activated platelets. mDia1 moved from a Triton-X100-soluble cytosolic fraction to insoluble cytoskeleton fraction after thrombin induced platelets aggregation. Anti-mDia1 antibody could inhibit this aggregation. PI3K inhibitor Wortmannin or Ly294002 inhibited the thrombin induced platelet aggregation and the above mentioned mDia1 translocation.
CONCLUSIONPI3-kinase mediates the thrombin-induced platelet aggregation through mDia1 pathway.
