In vitro absorption mechanism of strychnine and the transport interaction with liquiritin in Caco-2 cell monolayer model.
- Author:
Jun-jun WANG
1
;
Xiao-huan LIAO
;
Min YE
;
Yong CHEN
Author Information
1. Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine, Hubei University, Wuhan 430062, China.
- Publication Type:Journal Article
- MeSH:
ATP-Binding Cassette, Sub-Family B, Member 1;
antagonists & inhibitors;
Absorption;
drug effects;
Biological Transport;
drug effects;
Caco-2 Cells;
Flavanones;
isolation & purification;
pharmacology;
Glucosides;
isolation & purification;
pharmacology;
Glycyrrhiza uralensis;
chemistry;
Humans;
Hydrogen-Ion Concentration;
Permeability;
Plants, Medicinal;
chemistry;
Strychnine;
isolation & purification;
pharmacokinetics;
Strychnos nux-vomica;
chemistry;
Verapamil;
pharmacology
- From:
Acta Pharmaceutica Sinica
2010;45(9):1160-1164
- CountryChina
- Language:Chinese
-
Abstract:
To study the effect of liquiritin (Liq) on the transport of strychnine (Str) in Caco-2 cell monolayer model, the transport parameters of Str, such as apparent permeability coefficient (P app (B-->A) and P app (A-->B)) and cumulative transport amount (TRcum), were determined and comparatively analyzed when Str was used solely and co-used with Liq. The effect of drug concentrations, conveying times, P-glycoprotein (P-gp) inhibitor verapamil and conveying liquor pH values on the transport of Str were also investigated. The results indicated that the absorption of Str in Caco-2 cell monolayer model was well and the passive transference was the main intestinal absorption mechanism of Str in the Caco-2 monolayer model, along with the excretion action mediated by P-gp. Liq enhanced the absorption of Str. Meanwhile, conveying liquor pH value had significant influence on the excretion transport of Str.
