An experimental study of inhibiting effect of decoy oligodeoxynucleotides on the gene expression of collagen in NIH3T3 cell.
- Author:
Tian-zhi HAO
1
;
Huan-ping LIANG
;
Feng-lin LV
;
Xiang XU
;
Hua GUO
;
Fu-long WANG
;
Wen-jun YANG
;
Yan LUO
;
Lei LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Collagen Type I; biosynthesis; Fibroblasts; drug effects; metabolism; Mice; NIH 3T3 Cells; Oligodeoxyribonucleotides; genetics; pharmacology; RNA, Messenger; metabolism; Transcription Factor AP-1; genetics; Transfection
- From: Chinese Journal of Burns 2003;19(3):175-178
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of activator protein-1 (AP-1) decoy-oligodeoxynucleotides (Decoy-ODNs) on the expression of fibroblast alpha2 type I collagen, so as to explore the gene therapy of pathologic scar.
METHODSDecoy-ODNs targeting AP-1 were designed and synthesized. NIH3T3 cells were transfected by cationic liposomes. The distribution of Decoy-ODNs in the cells was investigated. The inhibiting effects of Decoy-ODNs on AP-1 were determined by electrophoretic mobility shift assay (EMSA). And the effects of Decoy-ODNs on the collagen synthesis in the cells were analyzed by RT-PCR.
RESULTSAP-1 Decoy-ODNs could competitively inhibit the AP-1 in vitro activity. Cationic liposomes could play roles by effectively transfecting Decoy-ODNs into the plasma and nucleus. The mRNA expression of fibroblast alpha2 type I collagen decreased evidently after 24 hours of Decoy-ODNs action.
CONCLUSIONDecoy-ODNs could inhibit the mRNA expression of fibroblast alpha2 type I collagen by antagonizing AP-1.
