Determination of fraxinellone in root bark of Dictamnus dasycarpus by RP-HPLC.
- Author:
Chun-lan YUAN
1
,
2
;
De-suo YANG
Author Information
- Publication Type:Journal Article
- MeSH: Benzofurans; analysis; Chromatography, High Pressure Liquid; methods; Dictamnus; chemistry; Plant Roots; chemistry; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2006;31(12):992-994
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a RP-HPLC method for determination of fraxinellone in Dictamnus dasycarpus.
METHODRP-HPLC conditions were as follows: AT-LICHROM C18(4.6 mm x 250 mm) column, MeOH-H2O (70:30) as a mobile phase, detection wavelength 240 nm, column temperature 25 degrees C, Flow velocity 0.5 mL x min(-1).
RESULTLinear relationship is very good (r = 0.9999) in 0.018-0.18 mg x mL(-1). The average recovery is 98.9%, RSD is 1.2% (n = 4).
CONCLUSIONThe method is simple and repeatable and can be used for quantitative analysis of fraxinellone in root bark of D. dasycarpus.
