Hydroxysafflor yellow A inhibits rat vascular smooth muscle cells proliferation possibly via blocking signal transduction of MEK-ERK1/2
10.3760/cma.j.issn.0253-3758.2015.08.017
- VernacularTitle:羟基红花黄色素A可抑制大鼠血管平滑肌细胞增殖
- Author:
Jingshan ZHAO
1
;
Qianyu GUO
;
Shaohong LAI
;
Shangyue ZHANG
;
Hongli HAN
Author Information
1. 河北中医学院基础医学院
- Keywords:
Muscle,smooth,vascular;
Cell proliferation;
Hydroxysafflor yellow A
- From:
Chinese Journal of Cardiology
2015;43(8):728-731
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the effect of hydroxysafflor yellow A (HYSA) on the proliferation of vascular smooth muscle cells (VSMCs) and the related mechanism.Methods VSMCs derived from SD rats were treated with DMEC culture medium (Control),10 ng/ml PDGF (PDGF group),pretreatment with HYSA at different doses (1,5,10,20,40,60 μmol/L) for 24 h then cotreatment with PDGF.After 24 h,MTT assay,Western blot and immunohistochemical staining were performed to evaluate the inhibitory effects of HYSA on VSMCs proliferation.Results HYSA inhibited PDGF induced VSMCs proliferation in a dose-dependent manner,dowregulated proliferating cell nuclear antigen (PCNA) expression and blocked PDGF activated PDGFR-MEK-ERK1/2 signaling pathway.Conclusions HYSA inhibits VSMCs proliferation possibly via downregulating the expression of PCNA and blocking MEK-ERK1/2 signal transduction in VSMCs.
