Expression of porcine interleukin-18 in baculovirus/insect cells.
- Author:
Zhenya WANG
1
;
Yanbin WANG
;
Hongying CHEN
;
Panfeng SHAO
;
Xiaodong NING
;
Na PAN
;
Lijing HAN
;
Baoan CUI
Author Information
1. College of Animal Husbandry and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Baculoviridae;
genetics;
metabolism;
Cells, Cultured;
Genetic Vectors;
genetics;
Insecta;
genetics;
metabolism;
Interleukin-18;
biosynthesis;
genetics;
Recombinant Proteins;
biosynthesis;
genetics;
immunology;
Swine;
Transfection
- From:
Chinese Journal of Biotechnology
2011;27(1):118-123
- CountryChina
- Language:Chinese
-
Abstract:
IL-18, as a polyphonic cytokine, is important in immune response and physiologic function. We designed one pair of primers, amplified the porcine IL-18 gene fused with a C-terminal 6xHistidine tag, and then subcloned into the pFastBacDual of Baculovirus transfer vector and transformed into DH10Bac containing a shuttle vector of Bacmid. After co-transfecting the recombinant plasmid into insect cells, the 18 kDa expressed protein of porcine IL-18 was detected by SDS-PAGE; the specificity of expressed protein was confirmed by Western blotting. The purified porcine IL-18 protein induced obvious proliferation of porcine T lymphocytes in vitro, which indicated that the expression of IL-18 had high biological activity.
