Separation and authentication of tilianin and quality standards of semen of Dracocephalum moldavia.
- Author:
Xue-mei CHENG
;
Ting-yun MA
;
Su LEY-MAN
;
Ha-Lik
;
Dan-dan MU
;
Tiann FANG
;
Gui-Xin CHOU
;
Zheng-tao WANG
;
Chang-hong WANG
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
Chromatography, Thin Layer;
Drugs, Chinese Herbal;
chemistry;
isolation & purification;
standards;
Flavonoids;
chemistry;
isolation & purification;
standards;
Glycosides;
chemistry;
isolation & purification;
standards;
Lamiaceae;
chemistry;
Magnetic Resonance Spectroscopy;
Quality Control
- From:
China Journal of Chinese Materia Medica
2015;40(10):1845-1849
- CountryChina
- Language:Chinese
-
Abstract:
Tilianin was separated and authenticated from the seeds of Dracocephalum moldavia, a Uygur medicine, by chromatographic technique and spectroscopic method. The purity of tilianin is more than 98% determined by HPLC area normalization method. Thin layer chromatography (TLC) method was used to separate tilianin from D. moldavia by mixture of chloroform-methanol (5: 1) as a developing solvent on high performance silicagel precoated plate (SGF254) and using aluminium trichloride as a chromogenic agent for qualitative identification of D. moldavia. To establish a HPLC method for quantitative analysis of D. moldavia, tilianin was used as a Quantitative marker and separated on a C18 (4.6 mm x 250 mm, 5 μm) column with acetonitrile-01% formic acid (25: 75) as the mobile phase and detected at 330 nm. The calibration curve of tilianin displayed ideal linearity over the range of 0.617 2-123.44 μg x mL(-1) with a regression equation of Y = 33.773X - 0.824 8 (r = 1). The average recovery of tilianin was 101.0% with RSD of 3.7%. The RSD values of intra-day and inter-day precision were less than 2%. The content of tilianin in 4 batches of the authenticated semen of D. Moldavia was between 0.016 and 0.187 mg x g(-1). The qualitative and quantitative method established is suitable for the quality evaluation and assessment of semen of D. Moldavia.
