Research of DNA barcode of Teseudinis Carapax et Planstrum and its adulterants based on COI gene sequence.
- Author:
Xiao-Fan LIU
1
;
Chun-Sheng LIU
;
Yao-Jun YANG
;
Jun-De LI
;
Jia XU
;
Li-Jie WU
;
Dai DAI
;
Jie LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Base Sequence; DNA Barcoding, Taxonomic; Electron Transport Complex IV; genetics; Medicine, Chinese Traditional; standards; Molecular Sequence Data; Phylogeny; Quality Control; Reptilian Proteins; genetics; Sequence Analysis, DNA; Turtles; classification; genetics
- From: China Journal of Chinese Materia Medica 2013;38(7):947-950
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo use COI gene on the Mauremys reevesii and its adulterants by molecular identification. Search a rapid, accurate method of identification of Teseudinis Carapax et Planstrum and its adulterants.
METHODWe collected 8 species of the authentic and adulterants of teseudinis carapax et planstrum in a nationwide then, extracted DNA, got the COI sequences. Use ContigExpress, Dnaman, Edit Sequence and Mega 5 to analyze the variable site and construct the N-J tree.
RESULTCompare with the authentic Teseudinis Carapax et Planstrum, the adulterant exist lots of variable site. The N-J tree Indicates that the same genus belong together and each species belong to relatively independent branch.
CONCLUSIONBased on the COI gene, the technology of DNA bar code can be a excellent identification of Teseudinis Carapax et Planstrum and its adulterants.
