Suppressed Production of Pro-inflammatory Cytokines by LPS-Activated Macrophages after Treatment with Toxoplasma gondii Lysate.
10.3347/kjp.2008.46.3.145
- Author:
Eun Jung LEE
1
;
Yoo Mi HEO
;
Jong Hak CHOI
;
Hyun Ouk SONG
;
Jae Sook RYU
;
Myoung Hee AHN
Author Information
1. Department of Parasitology and Institute of Biomedical Science, Hanyang University College of Medicine, Seoul 133-791, Korea. mhahn@hanyang.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Toxoplasma gondii;
LPS;
macrophage;
cytokine;
toll-like receptor;
NF-kappaB
- MeSH:
Animals;
Cell Line;
Cytokines/*biosynthesis;
Humans;
Inflammation/metabolism;
Lipopolysaccharides/*pharmacology;
Macrophages/*drug effects/*metabolism;
Toxoplasma
- From:The Korean Journal of Parasitology
2008;46(3):145-151
- CountryRepublic of Korea
- Language:English
-
Abstract:
During Toxoplasma gondii infection, macrophages, dendritic cells, and neutrophils are important sources of pro-inflammatory cytokines from the host. To counteract the pro-inflammatory activities, T. gondii is known to have several mechanisms inducing down-regulation of the host immunity. In the present study, we analyzed the production of proand anti-inflammatory cytokines from a human myelomonocytic cell line, THP-1 cells, in response to treatment with T. gondii lysate or lipopolysaccharide (LPS). Treatment of THP-1 cells with LPS induced production of IL-12, TNF-alpha, IL-8, and IL-10. Co-treatment of THP-1 cells with T. gondii lysate inhibited the LPS-induced IL-12, IL-8 and TNF-alpha expression, but increased the level of IL-10 synergistically. IL-12 and IL-10 production was down-regulated by anti-human toll-like receptor (TLR)-2 and TLR4 antibodies. T. gondii lysate triggered nuclear factor (NF)-kappaB-dependent IL-8 expression in HEK293 cells transfected with TLR2. It is suggested that immunosuppression induced by T. gondii lysate treatment might occur via TLR2-mediated NF-kappaB activation.
