UPLC-Q-TOF/MS for rapid analysis of chemical constituents in Sanhuang tablets.
10.19540/j.cnki.cjcmm.20170223.010
- Author:
Meng-Jiao LIU
1
;
Yun WANG
2
;
Ling-Yun LI
2
;
Xiao-Qing LI
2
;
Ye-Jia DAI
2
;
Xue ZHANG
2
;
Yin-Lian MA
2
;
Cun ZHANG
2
;
Wen-Lan LI
1
Author Information
1. Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
HPLC-MS;
Sanhuang tablets;
baicalin;
berberine;
rhubarb
- From:
China Journal of Chinese Materia Medica
2017;42(9):1685-1692
- CountryChina
- Language:Chinese
-
Abstract:
The qualitative analysis method of ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) was established for the chemical constituents in Sanhuang tablets. Waters ACQUITY BEH C₁₈ (2.1 mm×100 mm, 1.7 μm) column was used with 0.1% formic acid solution (A)-0.1% formic acid acetonitrile (B) as the mobile phase for gradient elution. The flow rate was 0.2 mL•min⁻¹; the sample volume was 1 μL and the column temperature was 30 ℃. The high-resolution quadrupole time-flight mass spectrometry was used as detector with electrospray ion source in both positive and negative models, and the dry gas temperature was 325 ℃. Based on the analysis of mass spectrometry and literature reports, 38 compounds were confirmed, including 1 alkaloid, 1 dianthrone compound, 6 tannins, 7 anthraquinone glycosides, 6 anthraquinones and 17 flavonoids. Liquid chromatography-mass spectrometry method is simple, reliable and rapid to identify the chemical compositions of Sanhuang tablets, and it is helpful to reveal its chemical constituents and pharmacodynamic substances.
