Analysis of Phosphatidylinositol 3-kinase Activation in the Adipose Tissue of Gestational Diabetes Mellitus Patients and Insulin Resistance
10.1007/s11596-010-0458-9
- Author:
CHU YONGLI
1
;
LIU WENJUAN
;
CUI QING
;
FENG GUIJIAO
;
WANG YAN
;
JIANG XUEQIANG
Author Information
1. Department of Obestetrics and Gynecology,Yantai Yuhuangding Hospital,Yantai 264000,China
- Keywords:
gestational diabetes mellitus;
insulin resistance;
phosphatidylinositol 3-kinase
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2010;30(4):505-508
- CountryChina
- Language:Chinese
-
Abstract:
The P85 regulatory subunit protein and gene expression and P110 catalylic subunit activity of phosphatidylinositol 3-kinase(PI-3K)were investigated in adipose tissue of patients with gestational diabetes mellitus(GDM)in order to explore the molecular mechanisms of insulin resistance(1R)of GDM.Samples from patients with GDM(n=50),and controls(n=50)were collected.Fasting insulin(FIN)was determined by radioimmunoassay.Fasting plasma glucose(FPG)was measured by oxidase assay.Western blot technique was used to detect the levels of PI-3K P85 subunit in adipose tissues of patients with GDM.The mRNA expression of PI-3K P85 subunit was detected by reverse transcription polymerase chain reaction(RT-PCR)method in the adipose tissue.PI-3K activity was examined by immunoprecipitation,thin-layer chromatography and gamma scintillation counting.The results were analyzed statistically.It was found that the levels of FPG,FIN and HOMA-IR in GDM group were significantly higher than those in control group(all P<0.01).There was no significant difference in the protein and gene expression of PI-3K P85 subunit between GDM group and control group(P>0.05).PI-3K activity was significantly decreased to 82.89% in GDM group as compared with control group(P<0.01)and negatively correlated with HOMA-IR(r=-0.75,P<0.01).It was concluded that PI-3K in GDM patients may be involved in the insulin signaling pathway,resulting in IR of GDM.
