Applications of platelet antigen gene typing and matching in the platelet transfusion.
10.7534/j.issn.1009-2137.2014.01.039
- Author:
Bing-Xian LIU
1
,
2
;
Yu-Jia LIU
3
;
Guang-Ping GAO
4
;
Dan WANG
4
;
Rui-Hua ZHOU
4
;
Qiang MA
4
;
Yan ZHANG
4
;
Xiu-Qing YU
4
;
Hua ZHENG
4
Author Information
1. Blood Bank of Mudanjiang, Mudanjiang 157011, Heilongjiang Province, China. E- mail:mdjlbx@
2. com.
3. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
4. Blood Bank of Mudanjiang, Mudanjiang 157011, Heilongjiang Province, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Antigens, Human Platelet;
genetics;
immunology;
Blood Grouping and Crossmatching;
Female;
Genotype;
Humans;
Male;
Middle Aged;
Platelet Transfusion;
methods;
Young Adult
- From:
Journal of Experimental Hematology
2014;22(1):199-203
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to establishment of known HPA genotype platelet donor data base and carry out the platelet antigen gene typing and matching in the platelet transfusion so as to prevent the production of the antibody and to avoid the platelet transfusion refractoriness. HPA of the donors and the patients were genotyped by PCR-SSP. The platelet transfusion with the same ABO type and HPA genotypes as the patient was selected and infused if the solid-phase agglutination matching was consistent. The results showed that the distribution of HPA types in Chinese Mudanjiang area had its own characteristics. HPA-15 had the greatest heterozygosity, and then HPA-3. The effective rate was 94.4% if both the HPA types and the ABO types were coincident while the effective rate was 77.8% when only ABO types were coincident. It is concluded that the distribution of HPA types in Chinese Mudanjiang area presents genetic polymorphism. The platelet transfusion with genetic typing and matching of both HPA and ABO types is an effective way to avoid production of the antibody and platelet transfusion refractoriness.
