Construction of tissue-engineering skin with carrier of active composite dermal matrix.
- Author:
Zhong-feng MA
1
;
Jia-ke CHAI
;
Hong-ming YANG
;
Li-ming LIANG
;
Ming-huo XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cattle; Cell Culture Techniques; Collagen Type I; Dermis; transplantation; Epidermis; cytology; Extracellular Matrix; transplantation; Fibroblasts; cytology; Humans; Skin; cytology; Skin, Artificial; Swine; Tissue Engineering
- From: Chinese Journal of Burns 2008;24(4):272-274
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct of tissue engineering skin including active composite dermal matrix.
METHODSThe human fibroblasts and bovine collagen with type I were inoculated on the surface of porcine acellular dermal matrix (PADM) for construction of active dermal substitute, then epidermal cells were inoculated on the dermal matrix for gas-liquid interface culture. The tissue-engineering skin was observed by histological examinations.
RESULTSThe structure of fibroblasts in collagen was intact, which was used to construct composite dermal matrix with PADM. The epithelial structure of tissue-engineering skin was similar to that of normal skin with good cell differentiation. Some phenomena were showed in epidermis: basic layer, stratum spinosum, granular layer and stratum corneum, desmosomes.
CONCLUSIONFibroblasts-Collagen-PADM can be an optimal dermal matrix for construction of tissue-engineering skin.
