Effects of domoic acid on membrane function of primary cultured rat glial cells.
- Author:
Lin-Lin LIU
1
;
Long LI
;
Dan CHEN
;
Ying-Sheng LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Membrane; drug effects; Cell Membrane Permeability; drug effects; Cells, Cultured; Kainic Acid; analogs & derivatives; pharmacology; Membrane Fluidity; drug effects; Neuroglia; drug effects; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2008;26(12):725-728
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of domoic acid (DA) on membrane function of primary cultured rat glial cell.
METHODSAfter the glial cells were treated with 6.4 x 10(-2), 6.4 x 10(-3) and 6.4 x 10(-4) micromol/L DA for 24 h, the activities of Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase, the membrane fluidity and the permeability were measured to reflect the membrane function.
RESULTSAfter treatment of DA for 24 h, the activities of Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase were inhibited significantly, the membrane fluidity decreased and the membrane permeability increased. The fluorescence polarization and microviscosity in the low, middle and high dosage treatment groups were 0.0626 +/- 0.0051, 0.0685 +/- 0.0097, 0.0648 +/- 0.0086 and 0.3154 +/- 0.0298, 0.3510 +/- 0.0571, 0.3286 +/- 0.0504 respectively, compared with the control group (0.0481 +/- 0.0069 and 0.2338 +/- 0.0372) (P < 0.01).
CONCLUSIONDA has obvious effects on membrane function of rat glial cells and may cause further injury on the cells.
