Hypoxia-A Possibility in Fibromyalgia Syndrome Pathogenesis.
- Author:
Seong Ho KIM
1
;
Il Soo MOON
Author Information
1. Department of Internal Medicine, Dongguk University, Gyeongju Hospital, Gyeongju, Korea. junjan@dongguk.ac.kr
- Publication Type:Original Article
- Keywords:
Fibromyalgia;
Hypoxia;
Substance P;
Glutamate;
Calcitonin gene related
- MeSH:
Animals;
Anoxia;
Calcitonin Gene-Related Peptide;
Carisoprodol;
Chronic Pain;
Embryonic Structures;
Fibromyalgia*;
Glutamic Acid;
Humans;
Immunohistochemistry;
N-Methylaspartate;
Rats;
Rats, Sprague-Dawley;
Receptors, Kainic Acid;
Substance P
- From:The Journal of the Korean Rheumatism Association
2006;13(3):218-225
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: We studied the expression of pain-related molecules such as substance P involved in chronic pain of fibromyalgia syndrome (FMS) patients using rat cortical cells in hypoxia. METHODS: We sacrificed pregnant Sprague-Dawley rat and got embryo. We cultured the cortical cells and compared the expression of pain-related molecules in 1st, 3rd, 5th day cortical cells exposed to hypoxia (37 degrees C, 5% CO2, 98% N2) to control by immunohistochemistry. We measured the density at soma using softwear 'Scion image'. RESULTS: The expression of substance P was increased in hypoxic cortical cell group than control (control mean: 49.9 vs. hypoxia 1st day: 75.4 (p<0.001), 3rd day: 65.6 (p<0.001), 5th day: 79.9 (p<0.001)). The expression of kainate receptor was increased in hypoxic cortical cell group than control (control mean: 58.4 vs. hypoxia 1st day: 64.9 (p<0.001), 3rd day: 63.3 (p<0.001), 5th day: 62.9 (p<0.001)). The expression of N-methyl-D-aspartate receptor 2B was increased in hypoxic cortical cell group than control (control mean : 59.4 vs. hypoxia 1st day: 60.8 (p<0.001), 3rd day: 62.6 (p<0.001), 5th day: 67.1 (p<0.001)). But, the expression of calcitonin gene related peptide was decreased in hypoxic cortical cell group than control (control mean: 76.8 vs. hypoxia 1st day: 76.4 (p<0.001), 3rd day: 71.5 (p<0.001), 5th day: 61.3 (p<0.001)). CONCLUSION: Hypoxia during night could increase the expression of some pain-related molecules, which might be the cause of chronic pain in FMS patients.
