Effect of NF-κB on proliferation of rat pulmonary artery smooth muscle cells inhibited by simvastatin.
- Author:
Zhong-Qiang LIU
1
,
2
;
Xiao-Qin WANG
;
Han-Min LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; drug effects; Hydroxymethylglutaryl-CoA Reductase Inhibitors; pharmacology; Male; Muscle, Smooth, Vascular; cytology; Myocytes, Smooth Muscle; physiology; NF-kappa B; analysis; genetics; physiology; Pulmonary Artery; cytology; RNA, Messenger; analysis; Rats; Rats, Sprague-Dawley; Simvastatin; pharmacology
- From: Chinese Journal of Contemporary Pediatrics 2015;17(2):185-189
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of NF-κB on proliferation of rat pulmonary artery smooth muscle cells (PASMC) inhibited by simvastatin.
METHODSPASMC isolated from rats and cultured in vitro were randomly divided into four groups (n=6 each): control, platelet-derived growth factor (PDGF) treatment, PDGF+simvastatin treatment, and PDGF+simvastatin+parthenolide (NF-κB inhibitor) treatment. MTT colorimetric assay and flow cytometry were performed to detect cell proliferation and cell cycle distribution. Immunohistochemistry was performed to detect the expression of NF-κB protein. Real-Time PCR was performed to detect NF-κB mRNA expression.
RESULTSCompared with the control group, MTT values of PASMC at all time points, cell proportion at the S phase and G2+M phase, NF-κB protein and mRNA expression increased significantly in the PDGF group (P<0.05). With the intervention of simvastatin, the levels of above indexes decreased compared with the PDGF group (P<0.05). With the intervention of simvastatin and parthenolide, the levels of above indexes decreased more obviously, but were not significantly different from those in the simvastatin intervention group.
CONCLUSIONSSimvastatin can inhibit proliferation of PASMC and cell cycle process. NF-κB may play an important role in the inhibitory effect of simvastatin on the proliferation of PASMC.
