Effect of transcription activity regulated by VNTR-ZNF and -14C/T variants in the promoter region of ATP-binding cassette transporter 1 in HepG2 cells.
- VernacularTitle:ABCA1基因启动子区VNTR-ZNF和-14nt位点变异对HepG2细胞中基因转录活性的影响
- Author:
Shenxia GAO
1
,
2
;
Lili ZHAO
;
Ying ZHANG
;
Yongmin MAO
Author Information
- Publication Type:Journal Article
- MeSH: ATP Binding Cassette Transporter 1; genetics; Base Sequence; Gene Expression Regulation, Neoplastic; Hep G2 Cells; Humans; Luciferases; genetics; metabolism; Minisatellite Repeats; genetics; Polymorphism, Single Nucleotide; Promoter Regions, Genetic; genetics; Zinc Fingers; genetics
- From: Chinese Journal of Medical Genetics 2016;33(5):633-636
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of VNTR-ZNF and -14C/T variants of the promoter region of the ABCA1 gene on the transcription activity of genes in vitro.
METHODSThe recombinants were constructed by ligating DNA fragment containing VNTR-ZNF ACCCC inserted/deleted allele with or without -14C/T substitution fragments with a PGL2-basic vector containing luciferase reporter gene. The recombinants were then transfected into HepG2 cells using the cationic lipid method. After 48 h, transfected cells were collected and used to detect the luciferase activity.
RESULTSLuciferase activity of PGL2-ZNF-ACCCCDel was greater than that of PGL2-ZNF-ACCCCIns. Luciferase activity of PGL2-ZNFDel-14C was greater than that of PGL2-ZNFDel-14T, PGL2-ZNFIns-14C, PGL2-ZNFIns-14T.
CONCLUSIONCompared with the insertion type, the ACCCC-deleted type of VNTR-ZNF can significantly enhance the transcription activity of ABCA1. And co-transfection of -14 C allele can further enhance this activity.
