Detection of single nucleotide polymorphisms of Jak2 gene in multiple myeloma by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
- Author:
Yue-Jiao ZHOANG
1
;
Bao-An CHEN
;
Lu CHENG
;
Ji-Feng FENG
;
Yu-Feng LI
;
Jun QIAN
;
Jia-Hua DING
;
Jian CHENG
;
Feng GAO
;
Guo-Hua XIA
;
Ning SUN
;
Yan ZHANG
;
Xiao-Ping ZHANG
;
Pei-Pei XU
;
Zu-Hong LU
Author Information
1. Department of Hematology, Zhongda Hospital, Southeast University Clinical Medical College, Nanjing 210009, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Case-Control Studies;
DNA Primers;
Genotype;
Humans;
Janus Kinase 2;
genetics;
Multiple Myeloma;
genetics;
Polymorphism, Single Nucleotide;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization;
methods
- From:
Journal of Experimental Hematology
2009;17(5):1234-1237
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the practicality of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) for detection of single nucleotide polymorphisms (SNP) on jak2 gene in multiple myeloma (MM) cells. The DNA fragment containing 2 SNPs of jak2 (C428T and C643T) was amplified using PCR and was purified. The purified product was used as the template for primer extension (PEX). The small products of allele-specific reaction were purified, the SNPs on jak2 gene of 5 patients with MM and 5 healthy persons were detected by MALDI-TOF MS. The results showed that the distribution of genotype C428T and C643T was not different between MM patients and healthy persons, both of which are homozygous T/T. In conclusion, the method based on MALDI-TOF MS and PEX technique for detecting SNP in jak2 gene is rapid, accurate and reliable method, and can be used in clinical practice.
