Mutation of lambdapL/pR-cI857 system for production of bacterial ghost in Escherichia coli.
- Author:
Hongliang DONG
1
;
Xian'gan HAN
;
Hao BAI
;
Liang HE
;
Lei LIU
;
Rui LIU
;
Tongjie CHAI
;
Chan DING
;
Haiwen LIU
;
Shengqing YU
Author Information
1. School of Animal Science and Technology, Shandong Agricultural University, Taian 271100, Shandong, China.
- Publication Type:Journal Article
- MeSH:
Bacteriolysis;
physiology;
Bacteriophage lambda;
genetics;
Base Sequence;
Cell Membrane;
physiology;
DNA, Bacterial;
analysis;
Escherichia coli;
genetics;
growth & development;
physiology;
virology;
Gene Expression Regulation;
genetics;
Molecular Sequence Data;
Mutation;
Promoter Regions, Genetic;
genetics;
Viral Proteins;
genetics;
metabolism
- From:
Chinese Journal of Biotechnology
2012;28(12):1423-1430
- CountryChina
- Language:Chinese
-
Abstract:
Bacterial ghost is intact envelope of Gram-negative bacteria, which is produced by the function of the lysis gene E from bacteriophage PhiX174. The expression of the lysis gene E is usually controlled by the thermosensitive lambdapL/pR-cI857 promoter. In this study, we described a mutation (T --> C) at the ninth nucleotide of the OR2 in the lambdapR promoter of the lambdapL/pR-cI857 system by overlap PCR. The bacteriolytic assay showed that the mutation in the lambdapL/pR-cI857 system enhanced the temperature of repressing the expression of gene E up to 37 degrees C. The lysis efficiency of altered lambdapR promoter in Escherichia coli DH5a and avian pathogenic E. coli DE17 was up to 99.9%. The expanded range of temperature will benefit for the production of bacterial ghost.
