Construction of eukaryotic expression vector of EGFRi-IL-24 recombinant gene.
- Author:
Jianling WANG
1
;
Xinying FAN
;
Leyuan BAO
;
Lianxiang DU
Author Information
1. Tianjin Key Lab of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Genetic Vectors;
genetics;
Humans;
Interleukins;
biosynthesis;
genetics;
Pichia;
genetics;
metabolism;
Receptor, Epidermal Growth Factor;
antagonists & inhibitors;
biosynthesis;
genetics;
Recombinant Fusion Proteins;
biosynthesis;
genetics;
pharmacology
- From:
Journal of Biomedical Engineering
2010;27(2):395-399
- CountryChina
- Language:Chinese
-
Abstract:
The epithelial growth factor receptor interference (EGFRi) was obtained by synthetic primers. Overlapping PCR was used to produce EGFRi-IL-24 fusion gene, which is linked by Gly4Ser3. After sequence analysis, EGFRi-IL-24 was cloned into expression vector pPIC9k; EGFRi-IL-24/pPIC9k was linearized with SacI,and then transformed to electroporated pastoris GS115. Subsequently, positive clone was selected by G418 and PCR, and its phenotype was determined by SDS-PAGE and MTT assay. The results demonstrated that EGFRi-IL-24 protein was expressed and shown to have the potential for use in researches of its biological function and in clinical application.
