Overexpression of PEMT2 inhibits the phosphorylation and translocation of PLC gamma 1.
- Author:
Ya-li LI
1
;
Yan-hua SHAO
;
Zhi-li LIU
;
Quan XIA
;
Ke-li MA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Cell Movement; Cell Proliferation; Liver Neoplasms, Experimental; Phosphatidylethanolamine N-Methyltransferase; genetics; metabolism; Phospholipase C gamma; genetics; metabolism; Phosphorylation; Proto-Oncogene Proteins c-met; metabolism; Rats; Transfection
- From: Chinese Journal of Hepatology 2006;14(7):514-516
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo explore the mechanism of CBRH-7919 cell proliferation inhibition by transfecting phosphatidylethanolamine N-methyltransferase 2 gene (PEMT2).
METHODSThe effects of PEMT2 transfection on phosphorylation and translocation from cytosol to plasma membrane of PLC gamma 1 in cells were studied using SDS-PAGE and Western blot techniques. The phosphorylation and activity of c-Met were determined.
RESULTSAfter transfection of pemt2, the PLC gamma 1 and phosphorylated PLC gamma 1 conjugated with plasma membrane were decreased by 45% and 27% of that of control cells respectively, and the phosphorylated c-Met was decreased to 32% of that of control cells.
CONCLUSIONTransfection of phosphatidylethanolamine N-methyltransferase 2 gene can inhibit the phosphorylation and translocation from cytosol to plasma membrane of PLC gamma 1 in cells. At the same time, the autophosphorylation of c-Met was decreased, which suggests that transfection of phosphatidylethanolamine N-methyltransferase 2 gene can downregulate the c-Met/PLC gamma 1 signaling pathway in CBRH-7919 cells.
