Change Profiles in Matrix Metalloproteinase-2 and-9 in Induced Endometriosis in Mice
10.1007/s11596-010-0211-4
- Author:
CHEN QIONGHUA
1
;
QIU NAXUAN
;
PU DEMIN
;
ZHOU YUMIN
;
LI TIAN
;
YANG HONGYI
Author Information
1. Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;Department of Gynecology and Obstetrics, the First Affiliated Hospital of Xiamen Medical University, Xiamen 361004, China
- Keywords:
matrix metalloproteinase;
endometriosis;
animal model
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2010;30(2):188-192
- CountryChina
- Language:Chinese
-
Abstract:
To examine the changes in matrix metalloproteinase-2(MMP-2)and-9(MMP-9)in the development and progression of endometriosis,real time quantitative polymerase chain reaction,enzyme-linked immunoabsorbent assay and gelatin zymography were employed to determine the mRNA and protein levels and activities of MMP-2 and MMP-9 from the first day to the 21st day after the induction in mice with induced endometriosis(experimental group)and sham-operated animals (controls).The results showed that the mRNA and protein levels and activities of the MMP-2 and MMP-9 were significantly increased on the first day after the induction and the level of MMP-2 stayed at a level higher than that in the control group.MMP-9 had two or three peaks during the 21 days,taking place at day 1,4 and 15.It is concluded that the changes in the MMP-2 and MMP-9 might be involved in pathogenesis of endometriosis.
