Reversal effect of cinobufacini on multidrug resistance of Raji/ADR cells and its mechanisms.
10.7534/j.issn.1009-2137.2014.05.023
- Author:
Cheng ZHANG
1
,
2
;
Ding-Ming WAN
3
;
Wei-Jie CAO
3
Author Information
1. Department of Hematology, The First Affiliated Hospital of Nanyang Medical College, Nanyang 473000, Henan Province, China. E-mail: physicianzc@
2. com.
3. Department of Hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China.
- Publication Type:Journal Article
- MeSH:
ATP-Binding Cassette, Sub-Family B, Member 1;
Amphibian Venoms;
Bufanolides;
pharmacology;
Cell Line, Tumor;
Doxorubicin;
Drug Resistance, Multiple;
drug effects;
Drug Resistance, Neoplasm;
drug effects;
Humans;
Reverse Transcriptase Polymerase Chain Reaction
- From:
Journal of Experimental Hematology
2014;22(5):1306-1310
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to explore the reversing effect of cinobufacini on multidrug resistance of Raji/ADR cells and its mechanisms. The growth inhibitory rate, half inhibitory concentration (IC50), reversing multiples to adriamycin- resistance were detected by MTT, and the curve of growth inhibitory rate was drawn; the MDR-1 and MRP-1 gene transcription was determined by RT-PCR; the expressions of P-gp and MRP-1 proteins were assayed by Western blot and flow cytometry. The results showed that the inhibitory rates of cinobufacini on Raji and Raji/ADR cells at 72 h were 75.6% and 69.3% respectively, the IC50 were 3.9 mmol/L and 4.6 mmol/L without significant difference (P > 0.05). The reversing multiples to adriamycin-resistance were 255.7 multiples, the transcription of mdr-1 and mrp-1 genes and the expression of P-gp and MRP-1 proteins significantly decreased (P < 0.05) in Raji/ADR cells after the treatment with cinobufotalin. It is concluded that cinobufotalin can reverse the adriamycin-resistance in Raji/ADR cells and the expression of P-gp and MRP-1 proteins were down-regulated through the transcriptional pathway. The cinobufotalin is an effective reversal agent for the multidrug resistance of tumors.
