Correlation between expression of SIL-TAL1 fusion gene and deletion of 6q in T-cell acute lymphoblastic leukemia.
10.7534/j.issn.1009-2137.2014.06.002
- Author:
Qian WANG
1
;
Li-Li WU
1
;
Hai-Ping DAI
1
;
Na-Na PING
1
;
Chun-Xiao WU
1
;
Jin-Lan PAN
1
;
Jian-Nong CEN
1
;
Hui-Ying QIU
1
;
Su-Ning CHEN
2
Author Information
1. The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of the Ministry of Health, Collaborative Innovation Center of Hematology, Suzhou 215006, Jiangsu Province, China.
2. The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of the Ministry of Health, Collaborative Innovation Center of Hematology, Suzhou 215006, Jiangsu Province, China. E-mail: chensuning@sina.com.
- Publication Type:Journal Article
- MeSH:
Chromosome Deletion;
Chromosomes, Human, Pair 6;
Comparative Genomic Hybridization;
Humans;
Leukemia-Lymphoma, Adult T-Cell;
Oncogene Proteins, Fusion;
genetics;
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma;
genetics;
Reverse Transcriptase Polymerase Chain Reaction
- From:
Journal of Experimental Hematology
2014;22(6):1508-1513
- CountryChina
- Language:Chinese
-
Abstract:
The present study was designed to investigate the prevalence and clinical significance of SIL-TAL1 rearrangements in T-cell acute lymphoblastic leukemia (T-ALL). The incidence of SIL-TAL1 rearrangements was analyzed by nest real-time quantitative polymerase chain reaction (RT-PCR) in 68 patients with T-ALL. Karyotypic analysis was performed by conventional R-banding assay and array-based comparative genomic hybridization (array-CGH). The results showed that SIL-TAL1 rearrangements were identified in 10/26 (38.5%) pediatric and 2/42 (4.8%) adult T-ALL cases, which indicate a pediatric preference for SIL-TAL1 rearrangements in T-ALL. Two different transcripts were detected in 6/12(50%) T-ALL samples. Abnormal karyotypes were detected in 6 out of 11 cases (54.5%) and a deletion of the long arm of chromosome 6 was observed in 4 cases. Array-CGH results of 2 T-ALL cases with SIL-TAL1 rearrangement revealed that this fusion gene was resulted from a cryptic deletion of 1p32, and the overlap region of 6q deletion was 6q14.1-16.3. These cases with SIL-TAL1 fusion had a higher white blood cell (WBC) count and higher serum levels of lactate dehydrogenase (LDH) than cases without SIL-TAL1 fusion. It is concluded that SIL-TAL1 rearrangements are associated with loss of heterozygosity of chromosomal 6q, and SIL-TAL1-positive patients are younger than SIL-TAL1-negative patients. In contrast to the cases without SIL-TAL1 fusion, there are many adverse prognostic factors in the cases with SIL-TAL1 fusion, such as higher WBC count and higher LDH levels.
