Effects of yikunning on the expressions of bcl-2 and bax in rat ovaries during perimenopausal period.
- Author:
Qing-zhi CUI
1
;
Yan-jun TIAN
;
Xiao-hui XIAO
;
Hai-Xia WEN
;
Guang-mei ZHANG
;
Guo-yi LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drugs, Chinese Herbal; pharmacology; Female; Ovary; metabolism; Perimenopause; drug effects; physiology; Proto-Oncogene Proteins c-bcl-2; genetics; metabolism; RNA, Messenger; genetics; metabolism; Rats; Rats, Wistar; Up-Regulation; bcl-2-Associated X Protein; genetics; metabolism
- From: Chinese Journal of Applied Physiology 2010;26(2):177-180
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of Yikunning (YKN, Chinese Traditional Medicine) on the expressions of bcl-2 and bax in rat ovaries during perimenopausal period.
METHODSThirty female Wistar rats during perimenopausal period were selected by unforced aging. Then the rats were divided into 3 groups randomly: YKN group, Livial control group and Aged control group. Ten young female Wistar rats were selected as young control group. Intragastric administrations were conducted for 4 weeks once daily continuously. The expressions of Bcl-2 Bax mRNAs and proteins in rat ovaries were detected by RT-PCR and Western blot.
RESULTSThe levels of Bcl-2, Bax mRNAs and proteins in rat ovaries in YKN group were higher than those in Aged control group, which showed differences among them (P < 0.01). The Bcl-2/Bax mRNA rate and protein rate in rat ovaries in YKN group were higher than those in Aged control group, which showed differences among them (P < 0.05 or P < 0.01).
CONCLUSIONYKN could increase the expressions of Bcl-2, Bax mRNAs and proteins and up-regulate the Bcl-2/Bax mRNA rate, protein rate in rat ovaries during perimenopausal period, which might be one of the molecular mechanisms of YKN postponed the ovarian failure and cured perimenopausal syndrome.
