The ALV-A/B specific antibodies correlation between ELISA and IFA detection in chicken serum.
- Author:
Xue LI
1
,
2
;
De-Qing LI
;
Peng ZHAO
;
Zhi-Zhong CUI
Author Information
1. College of Animal Science and Technology, Shandong Agricultural University, Shandong Engineering Research Center for Animal Disease Control and Prevention, Taian 271018, China. lixue880209@
2. com
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Viral;
blood;
immunology;
Avian Leukosis;
diagnosis;
immunology;
virology;
Avian Leukosis Virus;
classification;
immunology;
isolation & purification;
Cell Line;
Chickens;
Enzyme-Linked Immunosorbent Assay;
methods;
Fluorescent Antibody Technique, Indirect;
methods;
Poultry Diseases;
diagnosis;
immunology;
virology;
Species Specificity
- From:
Chinese Journal of Virology
2012;28(6):615-620
- CountryChina
- Language:Chinese
-
Abstract:
To study the correlation between ELISA and IFA tests in detection of ALV-A/B antibody in chicken sera, ELSA S/P values and IFA titers for different serum samples were measured and statistically analyzed. The results indicated that there was a strong positive correlation between ELISA S/P values and IFA titers (r = 0.97435, P < 0.001). Because the positive correlation between ELISA and IFA was so strong and antibody positive rates were identical in two tests, it suggested that IFA could be used as a alternative method to replace ELISA kit when only limited numbers of samples to be tested to reduce the cost and increase the sensitivity.
