Complete genome sequence characteristics of human echovirus 9 strain isolated in Yunnan, China.
- Author:
Yan-Ju ZHU
1
,
2
;
Yue PAN
;
Jun-Ying CHEN
;
Ya-Ling LIU
;
Hai-Jing SHI
;
Hong-Wei LIAO
;
Qiang-Ming SUN
;
Shao-Hui MA
Author Information
1. Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China. 13953842661@
2. com
- Publication Type:Case Reports
- MeSH:
Base Sequence;
China;
Echovirus 9;
classification;
genetics;
isolation & purification;
Female;
Genome, Viral;
Humans;
Infant;
Molecular Sequence Data;
Phylogeny;
Viral Proteins;
genetics
- From:
Chinese Journal of Virology
2013;29(2):132-136
- CountryChina
- Language:Chinese
-
Abstract:
To analyze the genomic sequence characteristics of a human Echovirus 9(ECHO-9) strain isolated from a child with Hand-foot-mouth disease (HFMD) in Kunming, Yunnan Province, in 2010. The complete genome sequence of a human echovirus 9 strain, MSH-KM812-2010 was determined. As other human enterovirus, its genome was 7,424 nucleotides (nts) in length and encoded for 2,203 amino acids (aas). In comparison to other human enteroviruses, MSH-KM812-2010 strain had the highest homology with other strains of human echovirus 9 in structural genomic regions and more homologous to other serotypes of B specie than to human echovirus 9 in non-structural genomic regions. Phylogenetic analysis based on complete VP1 gene revealed that the sequences of human echovirus 9 segregated into three distinct clades A, B and C with more than 15. 0% diversity between clades. All Chinese isolates belonged to the same clade. RDP3 and Blast revealed evident recombination in non-structural genomic regions. This report is the first to, describe the complete genome of the human echovirus 9 in China and provide an overview of the diversity of genetic characteristics of a circulating human echovirus 9.
