Proliferation characteristics of a PK-15 cell-adapted strain of porcine parvovirus.
- Author:
Yun-Fei WU
1
,
2
;
Ling ZHU
;
Zhi-Wen XU
;
Meng-Jin FU
;
Lei CHEN
;
Ai-Guo YANG
;
Wan-Zhu GUO
Author Information
1. Animal Biotechnology Center, Ya'an 625014, China. wyf198674@
2. com
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Line;
Cricetinae;
Cytopathogenic Effect, Viral;
DNA, Viral;
genetics;
Female;
Haplorhini;
Humans;
Male;
Parvoviridae Infections;
virology;
Parvovirus, Porcine;
genetics;
physiology;
Real-Time Polymerase Chain Reaction;
Reproducibility of Results;
Sensitivity and Specificity;
Swine;
Viral Proteins;
genetics;
Virus Replication
- From:
Chinese Journal of Virology
2013;29(4):357-363
- CountryChina
- Language:Chinese
-
Abstract:
To study the proliferation characteristics of PPV in differently infected way and the variance of concentrations in different cells. A strain of porcine parvovirus(PPV) was adapted to PK-15 cells, and a Real-time fluorescent quantitative PCR (FQ-PCR) assay was developed based on the specific region of the NS1 gene of PPV to quantify the PPV. The FQ-PCR was used to measure the viral concentration of virus-infected cells by simultaneous or step by step inoculation and plot one-step growth curves. The proliferation characteristics of PPV strain in different cells lines (HeLa, MDBK, PK-15 ,ST, F81, BHK-21 and Marc-145) was also compared. The results showed the PK-15 cell -adapted strain of PPV produced CPE after 12 passages, and maintained stable CPE at the following 10 messages. The one-step growth curve showed that the virus concentration of simultaneous inoculation was higher than that of the step-by-step inoculation, and the proliferation cycle of step-by-step inoculation was shorter. The proliferation ability of PPV strain in different cells showed that CPE appeared first inPK-15, followed by ST, HeLa and MDBK, and the virus concentration was highest in ST, followed byPK-15, MDBK and HeLa. NO proliferation was observed in F81, BHK-21 and Marc-145 cells. These findings lay a material foundation for the basic researches on PPV and the development of vaccine.
