Effect of 17beta-estradiol on phosphorylcholine cytidylyltransferase activity from cultured rat lung explants.

Author: Cha-Xiang GUAN ¹ ; Fu-Wen ZHOU ; Zi-Qiang LUO ; Chang-Qing ZHANG ; Xiao-Qun QIN
Author Information

1. Department of Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Publication Type:Journal Article
MeSH: Animals; Calmodulin; metabolism; Choline-Phosphate Cytidylyltransferase; metabolism; Culture Media, Serum-Free; Estradiol; pharmacology; In Vitro Techniques; Lung; drug effects; enzymology; Male; Protein Kinase C; metabolism; Rats; Rats, Wistar
From: Chinese Journal of Applied Physiology 2003;19(1):57-59
CountryChina
Language:Chinese
Abstract:
AIMTo investigate the influence and mechanisms of 17beta-estradiol on the CTP: phosphorylcholine cytidylyltransferase (CCT) activity from cultured lung explants without serum.
METHODSWe detected the amount of [M-14C] choline incorporation into phosphatidylcholine so as to reflect CCT activity by liquid scintillation.
RESULTS(1) 17beta-estradiol increased the CCT activity in dose-dependence and time-dependence. (2) Both the protein kinase C inhibitor H-7 and calmodulin antagonist W-7 abolished the stimulatory effect of 17beta-estradiol (3 x 10(-6) mol/L) on the CCT activity.
CONCLUSION17beta-estradiol can increase CCT activity in cultured lung explants, its mechanism is related to protein kinase C and calmodulin.