Effects of p,p'-dDE on the expression of androgen-binding protein, transferrin and inhibin B mRNA in rat sertoli cells.
- Author:
Guo-Hong LIU
1
;
Chong WANG
;
Hong-Kai LIU
;
Ke-Di YANG
Author Information
- Publication Type:Journal Article
- MeSH: Androgen-Binding Protein; biosynthesis; genetics; Animals; Dichlorodiphenyl Dichloroethylene; toxicity; Inhibins; biosynthesis; genetics; Male; RNA, Messenger; genetics; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Sertoli Cells; drug effects; metabolism; Transferrin; biosynthesis; genetics
- From: National Journal of Andrology 2006;12(2):104-111
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore effects of p, p'DDE on the expression of androgen binding protein (ABP), transferrin (Tf) and inhibin B (INH B) mRNA in testis Sertoli cells of Sprague Dawley rats.
METHODSA method has been set up to obtain a large number of viable Sertoli cells from SD rats of 18-20 days of age. With a series of concentration p,p'-DDE (10, 30, and 50 micromol/L) co-incubating the Sertoli cells in vitro, the expression of ABP, Tf and INH B mRNA were determined by RT-PCR.
RESULTSa) With increase of the incubated p, p'-DDE, the expression of ABP mRNA in Sertoli cells went up while that of Tf and INH B dropped in a dose-dependent manner (P < 0. 05). b) The correlation analysis among ABP, Tf and INH B showed that negative relationships were found between ABP and Tf or INH B, respectively (r = - 0. 391 3, P = 0. 032 5; r = - 0.235 2, P = 0.0158), and that positive correlation was indicated between Tf and INH B (r =0.4516, P =0.0047).
CONCLUSIONp,p'-DDE is a reproductive toxicant which disrupts the transcription of ABP, Tf and INH B in rat Sertoli cells so as to result in reproductive dysfunction.
