Antagonistic effect of ginkgolide homologues on PAF-induced platelet aggregation and neuroprotective effect.
10.19540/j.cnki.cjcmm.2017.0206
- Author:
Jun XU
1
;
Kui-Long WANG
1
;
Ze-Yu CAO
1
;
Liang CAO
1
;
Zhen-Zhong WANG
1
;
Wei XIAO
1
Author Information
1. State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu Kanion Parmaceutical Co., Ltd., Lianyungang 222001, China.
- Publication Type:Journal Article
- Keywords:
Ca2+ overload;
antagonistic to PAF receptor;
apoptosis;
ginkgolide homologues;
neuroprotection
- From:
China Journal of Chinese Materia Medica
2017;42(24):4716-4721
- CountryChina
- Language:Chinese
-
Abstract:
To study the antagonistic effect of ginkgolide homologues on platelet-activating factor (PAF)-induced platelet aggregation and investigate its neuroprotective effect. PAF was used as a coagulant, and ginkgolides were added to the rabbit blood samples respectively. The inhibitory effect of each compound on platelet aggregation was detected by turbidimetry. In L-glutamate induced primary cortical neuron cell injury model, MTT assay was used to detect cell viability. Intracellular free Ca2+ concentration in neurons was measured by using the fluorescent Ca2+ indicator Fura-2 AM. Morphological observation and Hoechst 33258 staining were used to detect the inhibitory effect of ginkgolide on neuronal apoptosis. The results showed that the inhibitory effect on PAF-induced platelet aggregation activity in ginkgolide homologues was ginkgolide K (GK), ginkgolide B (GB), ginkgolide A (GA), ginkgolide C (GC), ginkgolide M (GM), ginkgolide J (GJ) and ginkgolide (GL) from high to low. GB and GK (1-100 μmol•L ⁻¹) could significantly reduce the cell damage caused by L-glutamate, with survival rate increasing, intracellular calcium concentration reducing and cell morphology restoring. This paper has identified the activities and characteristics of various compounds of ginkgolide homologues on PAF-induced platelet aggregation as well as its neuroprotective effect.
