Quality of realgar and its influencing factors based on toxicity.
10.19540/j.cnki.cjcmm.20171023.010
- Author:
La JIANG
1
;
Huan-Hua XU
2
;
Zhen-Hong JIANG
3
;
Shi-Han YANG
2
;
Qiao-Li SHI
4
;
Zeng-Chun MA
2
;
Yue GAO
1
;
Zhi-Wu CHEN
1
Author Information
1. Department of Pharmacology, Anhui Medical University, Hefei 230032, China.
2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
3. Jiangxi Province Key Laboratory of Molecular Medicine, Nanchang 330006, China.
4. State Key Laboratory of Drug Metabolism, Hematological Pharmacology Research Room, Institute of Transfusion Medicine, Beijing 100850, China.
- Publication Type:Journal Article
- Keywords:
quality control;
realgar;
soluble arsenic;
temperature;
water content
- From:
China Journal of Chinese Materia Medica
2017;42(24):4782-4787
- CountryChina
- Language:Chinese
-
Abstract:
The results of a toxicity analysis showed differences from those of the existing experimental data. Therefore, HPLC-ICP-MS was used to analyze the soluble arsenic content at different valences in realgar prepared with water grind processing, which were collected from 3 companies. The results showed that the free arsenic of the 3 companies did not exceed the limit of Chinese Pharmacopoeia. However, if the free arsenic was calculated based on the total value of As(Ⅲ) + As(Ⅴ), free arsenic of 1 company exceeded the limit of Chinese Pharmacopoeia. The method of determining free arsenic in Chinese Pharmacopoeia. was ancient Cai's arsenic detection method, which had a certain limitation and failed to effectively avoid the toxicity of remaining arsenics except for trivalent arsenic. Then, we examined the effects of water and temperature on the content and form of soluble arsenic in realgar. The results showed that the content of soluble arsenic increased with the rise of water content, and the form of soluble arsenic did not change, there were only As (Ⅲ) and As (Ⅴ); With the simple temperature factor, there was an increasing trend in the content of soluble arsenic in the samples, the maximum increment was As (Ⅲ) 2.489 mg•g⁻¹ and As (Ⅴ) 0.546 mg•g⁻¹; When water and temperature played an synergistic effect, the increase of soluble arsenic in the samples significantly changed, the maximum increment was As (Ⅲ) 23.690 mg•g⁻¹, As (Ⅴ) 0.468 mg•g⁻¹, respectively. Through comprehensive analysis, we believed that the quality of realgar was susceptible to water content and temperature. Both of the single effect of water content and the synergistic effect of water and temperature can significantly change the content of soluble arsenic in realgar, and the water content was a high-risk factor. In the current Chinese Pharmacopoeia 2015 version, the free arsenic detection method had limitations, hence new techniques shall be introduced; At the same time, realgar does not have a water content inspection item in the current pharmacopoeia, which shall be added. However, due to the limit of water content, more in-depth studies are required.
