Structural characterization of Glehniae Radix polysaccharides using partial acid hydrolysis-hydrophilic interaction liquid chromatography-mass spectrometry.
10.19540/j.cnki.cjcmm.20170928.008
- Author:
Bao-Xiang DU
1
;
Mei-Rong XIANG
1
;
Ye-Pei FU
1
;
Hai-Qiang JIANG
2
;
Li-Li GONG
2
;
Rong RONG
1
Author Information
1. School of Pharmacy, Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China.
2. Experience Center of Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China.
- Publication Type:Journal Article
- Keywords:
Glehniae Radix polysaccharides;
hydrophilic liquid chromatography;
mass spectrometry;
partial acid hydrolysis
- From:
China Journal of Chinese Materia Medica
2017;42(24):4814-4818
- CountryChina
- Language:Chinese
-
Abstract:
Water-soluble polysaccharides from traditional Chinese medicine have properties of complex structure and high molecular, resulting in hardly complete their structural characterization.However, a "bottom-up" approach could solve this problem.Glehniae Radix extract was extracted with hot water and then precipitated by 40% ethanol to obtain Glehniae Radix polysaccharides (RGP). Subsequently, a partial acid hydrolysis method was carried out and the effects of acid concentration, time and temperature on hydrolysis were investigated. Under the optimum hydrolysis condition (1.5 mol•L⁻¹ trifluoroacetic acid, 4 h, and 80 ℃), RGP were hydrolyzed to characteristic oligosaccharide fragments. Futher, a hydrophilic liquid chromatography- mass spectrometry method was used for the separation and structural characterization of the polysaccharide hydrolysates. According to MS and MS/MS analysis of several standard disaccharides, a method for determining the type of polysaccharide glycosidic linkage by mass spectrometry was established. The results showed that the polysaccharide hydrolysates were linear glucan containing 1, 4-glycosidic bonds. And gluco-oligosaccharides with the degrees of polymerization (DP) of 4-11 were obtained after partial acid hydrolysis.
