Determination of artemisinin in rat plasma with HPLC-mS and its application.
- Author:
Chuan FU
1
;
Jiying YU
;
Jing ZOU
;
Lin HE
;
Yun DONG
;
Yuan HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Artemisinins; pharmacokinetics; Chromatography, High Pressure Liquid; Mass Spectrometry; Rats
- From: China Journal of Chinese Materia Medica 2012;37(19):2964-2967
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method for determining artemisinin in rat plasma in vivo.
METHODHPLC-MS was adopted. Estazolam was selected as an internal standard (I.S.). The sample and I.S. were extracted using methyl tertbutyl ether and measured at m/z of 305 and 296, respectively.
RESULTWithin the linear range of 5-500 microg x L(-1), the ratio of artemisinin's peak area and I.S. peak area and the concentration showed good linearity, thus the minimum concentration was set to be 5 mictrog x L(-1).
CONCLUSIONThe methodology proved that the method is so suitable for determining the drug concentration in rat blood that it can be used for studying pharmacokinetics in animals.
