Effect of Shenxiong injection on inflammation injury of ischemia-reperfusion injury rats.
- Author:
Hui-fen ZHOU
;
Jin-chao AL
;
Hai-tong WAN
;
Yu HE
;
Yu-yan ZHANG
;
Tao ZHAO
;
Wei FU
;
Jie-hong YANG
- Publication Type:Journal Article
- MeSH:
Animals;
Drugs, Chinese Herbal;
administration & dosage;
Humans;
Intercellular Adhesion Molecule-1;
genetics;
immunology;
Interferon-alpha;
genetics;
immunology;
Interleukin-1beta;
genetics;
immunology;
Male;
Matrix Metalloproteinase 9;
genetics;
immunology;
Rats;
Rats, Sprague-Dawley;
Reperfusion Injury;
drug therapy;
genetics;
immunology
- From:
China Journal of Chinese Materia Medica
2015;40(12):2408-2412
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of Shenxiong injection on the inflammation injury of ischemia-reperfusion injury senile rats. Totally 84 Sprague-Dawley (SD) rats were randomly divided into six groups: the sham operation group, the model group, the Nimodipine group and the Shenxiong injection(low, middle, and high dosage) groups. The rat cerebral ischemia-reperfusion model was established through intraperitoneal injection for 3 d and middle cerebral artery occlusion (MCAO). Ater the reperfusion for 24 h, efforts were made to give neurological score, collect brains for TTC staining, detect tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) content in serum by enzyme-linked immunosorbent assay (ELISA) method and measure IL-1β, ICAM-1 and MMP-9 mRNA expressions in hippocampal area by Real-time PCR (RT-PCR). According to the results, Shenxiong injection could decrease the cerebral infarction volume, greatly improved the neurological function and reduce IL-1β, TNF-α, ICAM-1 and MMP-9 mRNA expressions and IL-1β and TNF-α contents. In conclusion, Shenxiong injection shows the significant protective effect on ischemia-reperfusion injury in rats. Its mechanism may be related to the inhibition of inflammatory factor expression.
