GLP-1 receptor activation effects the p38MAPK signal pathway in hepatic stellate cells.
- VernacularTitle:胰高糖素样肽-1受体激活对肝星状细胞p38MAPK信号通路的影响
- Author:
Lingkang WU
1
;
Youming LI
;
Yingchao LIU
;
Cuilan TANG
;
Feng WU
;
Liangliang SHI
;
Keda LU
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Glucagon-Like Peptide 1; analogs & derivatives; pharmacology; Glucagon-Like Peptide-1 Receptor; Hepatic Stellate Cells; metabolism; Humans; Liraglutide; MAP Kinase Signaling System; RNA, Messenger; Receptors, Glucagon; metabolism; p38 Mitogen-Activated Protein Kinases; metabolism
- From: Chinese Journal of Hepatology 2015;23(2):130-133
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of activation of the GLP-1 receptor on the p38MAPK signaling pathway in hepatic stellate cells (HSCs).
METHODSHSCs were isolated and identified according to morphological features; the levels of GLP-1R protein were determined by western blotting.The HSCs were randomly divided into a control grouP (normal saline treatment) and experimental grouP(liraglutide treatment); after 120 hours, the expression of p38MAPK mRNA was examined by RT-PCR and of phosphorylated (p)-p38MAPK protein was detected by western blotting.
RESULTSGLP-1R proteins were detected in the HSCs. Compared with the control group, the experimental group showed significantly decreased p38MAPK mRNA and p-p38MAPK protein (both P < 0.01).
CONCLUSIONThe p38MAPK signaling pathway could be down-regulated when GLP-1R is activated in HSCs.
