Effects of WIN 55,212-2 on IK Current in Cultured Trigeminal Ganglion Neurons of Rat
- Author:
Zhangyin MING
1
;
Yan TAN
;
Hui FU
;
Xuehong CAO
;
Jianping PAN
;
Benrong HU
;
Lieju LIU
;
Jizhou XIANG
Author Information
1. Tongji Medical College,Huazhong University of Science and Technology
- Keywords:
WIN 55,212-2;
trigeminal ganglion neuron;
IK current;
rat
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(2):124-126
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of WIN 55,212-2 on IK in cultured rat trigeminal ganglion (TG) neurons, whole-cell patch clamp techniques were used to record the IK before and after WIN 55,212-2 perfusion at different concentrations. 30 μmol/L WIN 55,212-2 markedly (35.7 %±7.3%, P＜0. 01, n=8) inhibited IK currents, and the currents were partially recovered after washing.30μmol/L WIN 55,212-2 also induced a significant depolarizing shift in conductance-voltage parameters (control: V0.5 =10.43 ± 4.25 mV, k= 16.27±3.86; WIN 55,212-2: V0. 5 =24.71±3.91mV, k =16.69±2.75; n = 8, P＜0.01 for V0. 5). 0.01μmol/L WIN 55,212-2 slightly (27.0 %± 7.9 %, P＜0.05, n=7) increased IK currents, but had no significant change in conductance voltage parameters (control: V0.5 =10. 74±5. 27 mV, k=17. 33±2. 96; WIN 55,212-2: V0.5 =11.06±2.05 mV, k=19. 69±6. 60; n=7, P＞0.05 for V0.5 and k). These results suggested that WIN 55,212-2 has dual action, which might be through different receptors.
