Determination of R(-)- isomer in repaglinide tablets by capillary electrophoresis.
- Author:
Xiao-wei YUAN
1
;
Jia-yi SUN
1
;
Shi-zhuo WANG
1
;
Xing-jie GUO
1
Author Information
- Publication Type:Journal Article
- MeSH: Carbamates; analysis; Electrophoresis, Capillary; methods; Piperidines; analysis; Stereoisomerism; Tablets
- From: Journal of Zhejiang University. Medical sciences 2014;43(2):145-149
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a capillary electrophoresis system for enantiomeric impurity test of repaglinide.
METHODSAn uncoated fused silica capillary (50 μm×50 cm, with an effective length of 41 cm) was used. The running buffer was composed of 30 mmol/L NaH2PO4 and 5 mg/ml carboxymethyl-β-cyclodextrin(pH 3.5).
RESULTSLinear range was 2.00-80.00 μg/ml (correlation coefficient was 0.9993). The average recovery rate was 92.5% to 105.0%.
CONCLUSIONThe method is simple, accurate and sensitive and it can be used for determination of enantiomeric impurities in repaglinide tablet.
